Pro‐urokinase and prekallikrein are both associated with platelets

FEBS Letters

Johnstone, Michael¹  Loza, Jean-Pierre¹  Pannell, Ralph¹  Gurewich, Victor¹ 

[1] Vascular Research Laboratory, Institute for the Prevention of Cardiovascular Disease, Deaconess Hospital, Harvard Medical School, 185 Pilgrim Road, Boston, MA 02215, USA

关键词: Pro-urokinase;    Prekallikrein;    Platelet;    Fibrinolysis;    Pro-UK;    pro-urokinase;    UK;    urokinase;    u-PA;    urokinase plasminogen activator;    PK;    prekallikrein;    t-PA;    tissue plasminogen activator;    PGE;    prostaglandin E1;    PRP;    platelet-rich plasma;    PPP;    platelet-poor plasma;    HTA;    HEPES Tyrodes-Albumin;    HMW;    high molecular weight;    LMW;    low molecular weight;    SDS-PAGE;    sodium dodecyl sulfate polyacrylamide electrophoresis;    PPA;    plasma prekallikrein activator;   

DOI  :  10.1016/0014-5793(93)80537-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The contact-dependent intrinsic pathway of fibrinolysis involving factor XII, prekallikrein (PK) and pro-urokinase (pro-UK) remains poorly understood. Casein autography of washed, intact platelets revealed both PK and pro-UK. Accordingly, platelets may mediate physiological thrombolysis by this pathway since factor XIIa activates PK and kallikrein activates pro-UK. Acid washing dissociated PK but not pro-UK from platelets. Exogenous pro-UK was specifically incorporated by platelets from the ambient fluid and similarly could not be dissociated from intact platelets. Therefore, platelets may also mediate an effect from therapeutically administered pro-UK by prolonging its half-life.

【 授权许可】

Unknown