Determination of the vitronectin binding site on plasminogen activator inhibitor 1 (PAI‐1)

FEBS Letters

Pannekoek, Hans¹  Gebbink, Raymond Klein¹  Preissner, Klaus T.²  van Meijer, Marja¹ 

[1] Department of Biochemistry (K1-159), Academic Medical Center, University of Amsterdam, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands;Haemostasis Research Unit, Kerckhoff-Klinik, Max-Planck-Gesellschaft, Bad Nauheim, Germany

关键词: Fibrinolysis;    PAI-1;    Vitronectin;    Thrombin;    BSA;    bovine serum albumin;    MoAb(s);    monoclonal antibody(ies);    PAI-1;    plasminogen activator inhibitor 1;    SDS-PAGE;    sodium dodecyl sulfate polycrylamide gel electrophoresis;    S.E.M.;    standard error of the mean;    t-PA;    tissue-type plasminogen activator;   

DOI  :  10.1016/0014-5793(94)00990-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Vitronectin is the carrier protein of plasminogen activator inhibitor 1 (PAI-1). We used a well-characterized panel of anti-human PAI-1 monoclonal antibodies (MoAbs) to localize the vitronectin-binding site on PAI-1. By employing a direct vitronectin/PAI-1 binding assay and two vitronectin-dependent inhibition assays, we demonstrate that the anti-PAI-1 MoAbs CLB-5, CLB-10, CLB-2C8 and I1, directed against different epitopes in the region between amino acids 110 and 145, prevent the interaction of PAI-1 with vitronectin. We conclude that the region between amino acids 110 and 145 of PAI-1 harbours an important determinant for the interaction with vitronectin.

【 授权许可】

Unknown