A protein molecule as a bioanalytical device

FEBS Letters

Morozov, Victor N.¹  Morozova, Tamara Ya.¹ 

[1] Institute of Biological Physics, Academy of Sciences of the USSR, Pushchino, Moscow Region 142292, USSR

关键词: Immobilized enzyme;    Binding assay;    Papain;    Elastic response;    Protein sensor;   

DOI  :  10.1016/0014-5793(84)80755-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Cross-linked protein solids are proposed as new types of chemomechanical sensors for identification of biological molecules and measurement of their concentration. New sensors make use of the ability of proteins to specifically bind its ligands and to alter their conformation upon the binding. The use of immobilized proteins enables these conformational changes to be detected through the changes in mechanical properties of protein solid samples (stress, stain or the Young's modulus). Some advantages of the new sensors over enzyme probes are illustrated on considering the properties of papain sensor for α-N-benzoyl-L-arginine ethyl ester.

【 授权许可】

Unknown