A novel approach to the study of glycolipid receptors for viruses

FEBS Letters

Karlsson, Karl-Anders³  Norrby, Erling¹  Thurin, Jan³  Larson, Göran³  Örvell, Claes²  Hansson, Gunnar C.³  Strömberg, Nicklas³ 

[1] Department of Virology, Karolinska Institutet, S-104 01 Stockholm, Sweden;Department of Virology, National Bacteriological Laboratory, S-105 21 Stockholm, Sweden;Department of Medical Biochemistry, University of Göteborg, PO Box 33031, S-400 33 Göteborg, Sweden

关键词: Sendai virus;    Glycolipid;    Receptor;    Binding assay;    Thin-layer chromatography;    Monoclonal antibody;   

DOI  :  10.1016/0014-5793(84)81359-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A method for the binding of virus to a silica gel thin-layer chromatogram is presented. After development the chromatogram is overlayed with the ¹²⁵I-labelled virus and the bound virus is autoradiographed. Alternatively, the unlabelled virus may be detected after exposure to monoclonal antibody and labelled anti-antibody. The Sendai virus strain used did not bind to brain gangliosides earlier proposed to be receptors, but bound to human erythrocyte gangliosides. This finding may be explained by the existence of Sendai virus variants with different receptor specificities.

【 授权许可】

Unknown