Interproton distance measurements in solution for a double‐stranded DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon

FEBS Letters

Clore, G.Marius¹  Gronenborn, Angela M.¹ 

[1] Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW7 1AA, England

关键词: DNA oligonucleotide;    NOE;    Interproton distance;    Solution structure;    CRP specific site;   

DOI  :  10.1016/0014-5793(84)80582-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Using the proton-proton nuclear Overhauser effect, a large number (150) of interproton distances, both intra- and internucleotide, are determined in solution for a non-self-complementary DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. It is shown that these distances are very similar to those expected for classical B DNA (RMS difference of 0.5 Å) but are significantly different from those expected for classical A DNA (RMS difference 1.1Å). Glycosidic (χ) and C4'-C3' (δ) bond torsion angles are obtained by model building on the basis of the intranucleotide interproton distances. Whereas the sugar pucker exhibits little base-to-base variation with δ lying in the range 120 ± 10°, the glycosidic bond torsion angles of the pyrimidine and purine residues are significantly different, with χ_pyr = −120 ± 10° and χ_pur = − 90 ± 10°.

【 授权许可】

Unknown