FEBS Letters | |
Interproton distance measurements in solution for a double‐stranded DNA undecamer comprising a portion of the specific target site for the cyclic AMP receptor protein in the gal operon | |
Clore, G.Marius1  Gronenborn, Angela M.1  | |
[1] Division of Physical Biochemistry, National Institute for Medical Research, Mill Hill, London NW7 1AA, England | |
关键词: DNA oligonucleotide; NOE; Interproton distance; Solution structure; CRP specific site; | |
DOI : 10.1016/0014-5793(84)80582-7 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
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【 摘 要 】
Using the proton-proton nuclear Overhauser effect, a large number (150) of interproton distances, both intra- and internucleotide, are determined in solution for a non-self-complementary DNA undecamer comprising a portion of the specific target site for the cAMP receptor protein in the gal operon. It is shown that these distances are very similar to those expected for classical B DNA (RMS difference of 0.5 Å) but are significantly different from those expected for classical A DNA (RMS difference 1.1Å). Glycosidic (χ) and C4'-C3' (δ) bond torsion angles are obtained by model building on the basis of the intranucleotide interproton distances. Whereas the sugar pucker exhibits little base-to-base variation with δ lying in the range 120 ± 10°, the glycosidic bond torsion angles of the pyrimidine and purine residues are significantly different, with χpyr = −120 ± 10° and χpur = − 90 ± 10°.
【 授权许可】
Unknown
【 预 览 】
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RO201912020285859ZK.pdf | 577KB | ![]() |