Determination of distances involving exchangeable protons from NOESY spectra of two DNA dodecamers

FEBS Letters

Russu, Irina M.¹  Michalczyk, Ryszard¹ 

[1] Department of Molecular Biology and Biochemistry, Wesleyan University, Middletown, CT 06459, USA

关键词: 2D NMR;    NOE;    DNA oligonucleotide;    Inter-proton distance constraint;    Exchangeable proton;    NMR;    nuclear magnetic resonance;    ID;    one-dimensional;    2D;    two-dimensional;    NOESY;    nuclear Overhauser effect spectroscopy;   

DOI  :  10.1016/0014-5793(93)80340-Z

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Two-dimensional proton nuclear Overhauser effect (NOESY) spectra were obtained as a function of mixing time for two DNA dodecamers, 5'-CGCGAATTCGCG-3' and 5'-CGCAAATTTGCG-3', in aqueous solutions. The time evolution of cross-peak volumes was quantitatively analyzed based on the approximate solution of the relaxation/exchange equation over a range of mixing times from 30 to 150 ms. Inter-proton distances, involving exchangeable protons in key locations of the structures, were calculated and compared to the distances predicted by the crystal structures. These NMR-derived distances enhance the number of constraints, and their accuracy, for determination of solution structures of the two DNA dodecamers.

【 授权许可】

Unknown