2D 1H NMR studies of oxidized 2(Fe4S4) ferredoxin from Clostridium pasteurianum

FEBS Letters

Messori, Luigi³  Luchinat, Claudio²  Vallini, Giovanni¹  Bertini, Ivano³  Briganti, Fabrizio³  Scozzafava, Andrea³  Monnanni, Roberto³ 

[1] Centro di Studio per la Microbiologia del Suolo, CNR, Pisa, Italy;Institute of Agricultural Cry, University of Bologna, Bologna, Italy;Department of Chemistry, University of Florence, Florence, Italy

关键词: Ferredoxin;    Tron-sulfur cluster;    2D NMR;    Metalloprotein;    CpFd;    Clostridium pasteurianum 2(Fe4S4) ferredoxin;    COSY;    correlation spectroscopy;    DSS;    2;    2-dimethyl-2-silapentane-5-sulfonate;    FID;    free induction decay;    NOESY;    nuclear Overhauser eftect spectroscopy;    NOE;    nuclear Overhauser effect;    RD;    recycle delay;    τm;    mixing time;   

DOI  :  10.1016/0014-5793(91)81082-J

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Oxidized ferredoxin from Clostridium pastetirianum, containing two Fe₄S₄ clusters, has been investigated using 2D ¹H NMR spectroscopy at 600 MHz. 2D NMR experiments allowed complete assignment of the sixteen isotropically shifted signals corresponding to the β-CH₂ protons of the eight metal coordinated cysteines. Geminal connectivities of Cys β-CH₂ protons were identified through magnitude COSY experiments and confirmed through 2D NOESY experiments. A few additional signals could be assigned to the corresponding α-CH protons. The importance of 2D experiments to achieve firm assignments of isotropically shifted signals in paramagnetic metalloproteins is stressed.

【 授权许可】

Unknown