期刊论文详细信息
FEBS Letters
Probing the three‐dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements
Clore, G.Marius1  Gronenborn, Angela M.1 
[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried bei München, FRG
关键词: Oligonucleotide;    NMR;    NOE;    Interproton distance;    Solution structure;   
DOI  :  10.1016/0014-5793(85)80516-0
学科分类:生物化学/生物物理
来源: John Wiley & Sons Ltd.
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【 摘 要 】

DNA and RNA oligonucleotides are ideally suited for high-resolution X-ray crystallographic and 1H-NMR studies. The solution structures of such oligonucleotides can potentially be solved using proton-proton nuclear Overhauser enhancement measurements to demonstrate the proximity of protons in space and to determine their separation, thereby enabling a comparison of the structure in the crystalline and solution states to be made. In this review we describe (i) the general strategy for the sequential resonance assignments of oligonucleotide 1H-NMR spectra, the essential prerequisite for further structural work, (ii) the approach to obtaining interproton distances from pre-steady state nuclear Overhauser enhancement measurements, and (iii) the use of interproton distances in structure determination. This is illustrated by several examples including double- and single-stranded DNA oligonucleotides as well as RNA stem and loop structures.

【 授权许可】

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