A simple method for delineating well‐defined and variable regions in protein structures determined from interproton distance data

FEBS Letters

Clore, G.Marius¹  Nilges, Michael¹  Gronenborn, Angela M.¹ 

[1] Max-Planck-Institut für Biochemie, D-8033 Martinsried bei München, FRG

关键词: Protein structure;    3D solution structure;    NMR;    Nuclear Overhauser effect;    Interproton distance;    NMR;    nuclear magnetic resonance;    NOE;    nuclear Overhauser effect;    NOESY;    two-dimensional NOE spectroscopy;    rms;    root mean square;   

DOI  :  10.1016/0014-5793(87)81181-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

A simple method is described for identifying well-defined regions in a set of protein structures calculated from experimental interproton distance restraints. Two different functions, one based on the mean global rms difference, the other on the distance variation between equivalent atoms in different residues, are used to distinguish ‘variable’ from ‘well-defined’ regions. These functions are calculated in an iterative manner. The method is also capable of identifying several locally well-defined regions whose relative positions are not well-defined globally.

【 授权许可】

Unknown