Nuclear Overhauser effects in linear peptides A low‐temperature 500 MHz study of Met‐enkephalin

FEBS Letters

Motta, A.¹  Temussi, P.A.²  Tancredi, T.¹ 

[1] CMIB del CNR, via Toiano 6, Arco Felice, Napoli Italy;Dipartimento di Chimica, Università di Napoli, via Mezzocannone 4, 80134 Napoli, Italy

关键词: Nuclear Overhauser effect;    Met5-enkephalin;    Cryoprotective mixture;    Conformation;    DMSO;    dimethylsulfoxide;    NOE;    nuclear Overhauser enhancement;    NMR;    nuclear magnetic resonance;    TSP;    sodium 3-trimethyisilyl propionate;   

DOI  :  10.1016/0014-5793(87)80148-5

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Met⁵-enkephalin was studied in 1 mM solutions in ²H₂O at room temperature and in a cryoprotective mixture (DMSO_d6/²H₂O, mole fraction of DMSO 0.49) in the temperature range 265–298 K. Small positive effects were observed between the ortho and meta protons of Tyr in aqueous solution at room temperature. Intraresidue effects can be made strong and negative by increasing the viscosity of the medium with a combination of cryoprotective mixtures and low temperatures. The use of mixtures with properties very close to water is very promising for conformational studies of enkephalins and of other small linear peptides.

【 授权许可】

Unknown