Subcellular localisation of cerebral fucosyltransferase

FEBS Letters

Peschard, Marie-Jo¹  Baubichon-Cortay, Hélène¹  Louisot, Pierre¹  Broquet, Pierre¹  Serres-Guillaumond, Mireille¹ 

[1] Department of General and Medical Biochemistry, University of Lyon, INSERM U.189 and ERA-CNRS 562, Lyon-Sud Medical School, BP 12, 69921 Oullins Cédex, France

关键词: Fucosyltransferase;    Cerebral fractionation;    Enzyme marker;   

DOI  :  10.1016/0014-5793(84)81074-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

GDP-fucose : asialofetuin fucosyltransferase from sheep brain was fractionated on a sucrose gradient into two activity peaks. Using purification on Ficoll adapted from the proposed method [(1980) J. Neurochem. 35, 281-296], double localisation of cerebral fucosyltransferase was confirmed and the subcellular active fractions identified as light microsomes and mitochondria.

【 授权许可】

Unknown