Lex Structure Enhances Myocardial Differentiation from Embryonic Stem Cells

Cell Structure and Function

Kenji Kadomatsu¹  Takashi Muramatsu¹  Hisako Muramatsu¹  Akiharu Sudou¹  Tadashi Kaname¹ 

[1] Department of Biochemistry, Nagoya University School of Medicine

关键词: Fucosyltransferase;    carbohydrate antigens;    embryonic stem cells;    myocardial cells;    integrin;   

DOI  :  10.1247/csf.22.247

学科分类：分子生物学，细胞生物学和基因

来源: Japan Society for Cell Biology

【 摘 要 】

References(18)Cited-By(3)α-1, 3-Fucosyltransferase (Fuc TIV) cDNA was placed under the control of β-actin, cytomegalovirus enhancer/promoter and transfected into embryonic stem cells. The transfected cell clones with integrated cDNA (positive clones) differentiated more efficiently into myocardial cells than the clones without integrated cDNA (negative clones) or parental cells. Furthermore, myocardial cells differentiated from the positive clones survived longer than those differentiated from the negative clones or parental cells. These results indicate that Lewis X structure, the product of Fuc T IV, enhances myocardial differentiation. The mechanism of the phenomenon is discussed in relation to integrin action.

【 授权许可】

Unknown