α1,3‐Fucoslytransferase IX (Fuc‐TIX) is very highly conserved between human and mouse; molecular cloning, characterization and tissue distribution of human Fuc‐TIX

FEBS Letters

Nakagawa, Satoshi¹  Kudo, Takashi⁴  Narimatsu, Hisashi⁴  Ikehara, Yuzuru⁴  Iwasaki, Hiroko⁴  Kaneko, Mika⁴  Nishihara, Shoko⁴  Shiina, Takashi³  Saitou, Naruya²  Inoko, Hidetoshi³  Sasaki, Katsutoshi¹ 

[1] Tokyo Research Laboratories, Kyowa Hakko Kogyo Company, Limited, 3-6-6 Asahi-machi, Machida-shi, Tokyo 194-8533, Japan;Department of Genetics, School of Life Science, Graduate University for Advanced Studies, National Institute of Genetics, 1111 Yata, Mishima-shi, Shizuoka 411-8540, Japan;Second Department of Molecular Life Science, School of Medicine, Tokai University, Isehara-shi, Kanagawa 259-1193, Japan;Division of Cell Biology, Institute of Life Science, Soka University, 1-236 Tangi-cho, Hachioji-shi, Tokyo 192-8577, Japan

关键词: Glycosyltransferase;    Fucosyltransferase;    α1;    3-Fucosyltransferase;    Lewis x;    CD15;    SSEA-1;    Fuc-T;    fucosyltransferase;    Lex;    Lewis x;    Fuc;    fucose;    GlcNAc;    N-acetylglucosamine;    CNS;    central nervous system;    sLex;    sialyl Lewis x;    ORF;    open reading frame;    PA;    pyridylamination;    RT-PCR;    reverse transcription-polymerase chain reaction;    Ley;    Lewis y;    LNnT;    lacto-N-neotetraose;    LNT;    lacto-N-tetraose;    sialyl-LNnT;    α2;    3-sialyl lacto-N-tetraose;    Lea;    Lewis a;    PBL;    peripheral blood leukocytes;   

DOI  :  10.1016/S0014-5793(99)00640-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The amino acid sequence of Fuc-TIX is very highly conserved between mouse and human. The number of non-synonymous nucleotide substitutions of the Fuc-TIX gene between human and mouse was strikingly low, and almost equivalent to that of the α-actin gene. This indicates that Fuc-TIX is under a strong selective pressure of preservation during evolution. The human Fuc-TIX (hFuc-TIX) showed a unique characteristics, i.e. hFuc-TIX was not activated by Mn²⁺ and Co²⁺, whereas hFuc-TIV and hFuc-TVI were activated by the cations. The hFuc-TIX transcripts were abundantly expressed in brain and stomach, and interestingly were detected in spleen and peripheral blood leukocytes.

【 授权许可】

Unknown