FEBS Letters | |
A fucosyltransferase in teratocarcinoma stem cells | |
Muramatsu, Takashi1  Muramatsu, Hisako1  | |
[1] Department of Biochemistry, Kagoshima University School of Medicine, 1208-1, Usukicho, Kagoshima 890, Japan | |
关键词: Fucosyltransferase; Glycosyltransferase; Teratocarcinoma stem cell; Cell surface; Differentiation; Parietal endoderm cell; Diff-F9; F9 cells differentiated by retinoic acid and dibutyryl cyclic AMP; FBP; fucose-binding proteins of Lotus tetragonolobus; | |
DOI : 10.1016/0014-5793(83)80814-X | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Teratocarcinoma stem cell F9 expressed a potent fucosyltransferase activity acting on asialofetuin. A majority of the product was susceptible to α-L-fucosidase I from almond emulsin, indicating that the linkage formed was mainly Fucα1 → 3GlcNAc. The specific activity of the transferase decreased when the stem cells were induced to differentiate into parietal endoderm cells by retinoic acid and dibutyryl cyclic AMP. Furthermore, PYS-2 cell, a parietal endoderm cell line virtually lacked the transferase. The change in the fucosyltransferase activity could be correlated with cell surface changes occurring during differentiation.
【 授权许可】
Unknown
【 预 览 】
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