A fucosyltransferase in teratocarcinoma stem cells

FEBS Letters

Muramatsu, Takashi¹  Muramatsu, Hisako¹ 

[1] Department of Biochemistry, Kagoshima University School of Medicine, 1208-1, Usukicho, Kagoshima 890, Japan

关键词: Fucosyltransferase;    Glycosyltransferase;    Teratocarcinoma stem cell;    Cell surface;    Differentiation;    Parietal endoderm cell;    Diff-F9;    F9 cells differentiated by retinoic acid and dibutyryl cyclic AMP;    FBP;    fucose-binding proteins of Lotus tetragonolobus;   

DOI  :  10.1016/0014-5793(83)80814-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Teratocarcinoma stem cell F9 expressed a potent fucosyltransferase activity acting on asialofetuin. A majority of the product was susceptible to α-L-fucosidase I from almond emulsin, indicating that the linkage formed was mainly Fucα1 → 3GlcNAc. The specific activity of the transferase decreased when the stem cells were induced to differentiate into parietal endoderm cells by retinoic acid and dibutyryl cyclic AMP. Furthermore, PYS-2 cell, a parietal endoderm cell line virtually lacked the transferase. The change in the fucosyltransferase activity could be correlated with cell surface changes occurring during differentiation.

【 授权许可】

Unknown