Effect of N‐acetylglucosaminyltransferase V on the expressions of other glycosyltransferases

FEBS Letters

Chen, Hui-li¹  Zhang, Xia-ying¹  Guo, Peng¹  Zhang, Ying¹  Shen, Zong-hou¹ 

[1] Key Laboratory of Glycoconjugate Research, Ministry of Health, Department of Biochemistry, Shanghai Medical College, Fudan University, Shanghai 200032, PR China

关键词: N-acetylglucosaminyltransferase V;    Sialyl Lewis X;    α1;    3-Fucosyltransferase;    Sialyltransferase;    Core 2-β1;    6-N-acetylglucosaminyltransferase;    Gal;    galactose;    GlcNAc;    N-acetylglucosamine;    GalNAc;    N-acetylgalactosamine;    SA;    sialic acid;    GnTV;    N-acetylglucosaminyltransferase V;    FucT;    fucosyltransferase;    ST;    sialyltransferase;    ST3Gal;    cytidine monophosphate-N-acetylneuraminate:β-galactoside α2;    3-sialyltransferase;    ST6Gal;    cytidine monophosphate-N-acetylneuraminate:β-galactoside α2;    6-sialyltransferase;    FITC;    fluorescein isothiocyanate;    FACS;    fluorescence-activated cell spectra;    RT-PCR;    reverse transcriptase-polymerase chain reaction;    MAA;    Maackia amurensis agglutinin;    SNA;    Sambucus nigra agglutinin;    HRP;    horseradish peroxidase;    PBS;    phosphate-buffered saline;   

DOI  :  10.1016/S0014-5793(04)00188-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Transfection of sense cDNA of N-acetylglucosaminyltransferase V (GnTV) into H7721 human hepatocellular carcinoma cells resulted in the decreased expression of surface sialyl Lewis X (SLe^x), a sialylated fucose-containing antigen. The enzymatic mechanisms were speculated to be the concomitantly decreased expression of α1,3-fucosyltransferase (FucT)-III, -VI, -VII and the branching enzyme of O-glycans, core 2-β1,6-N-acetylglucosaminyltransferase (C2GnT)-I, -II. These two glycosyltransferase families were suggested to be the key enzymes in the synthesis of SLe^x. The expression of α2,3-sialyltransferase (ST3)-IV, but not ST3-I, -II and -III was elevated by sense GnTV. However, it did not cause the increase of SLe^x synthesis. Transfection of antisense GnTV into H7721 cells showed entirely opposite effects on the expression of above-mentioned SLe^x and glycosyltransferases as the sense GnTV.

【 授权许可】

Unknown