Identification and characterization of ligands for L‐selectin in the kidney. II. Expression of chondroitin sulfate and heparan sulfate proteoglycans reactive with L‐selectin

FEBS Letters

Watanabe, Norifumi¹  Li, Yong-Fei¹  Kawashima, Hiroto¹  Miyasaka, Masayuki¹ 

[1] Department of Bioregulation, Biomedical Research Center, Osaka University Medical School 2-2, Yamada-oka, Suita 565-0871, Japan

关键词: L-selectin;    Kidney;    Chondroitin sulfate;    Heparan sulfate;    Proteoglycan;    Sialyl Lewis X;   

DOI  :  10.1016/S0014-5793(99)00046-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Ligands for the leukocyte adhesion molecule L-selectin are expressed not only in lymph node high endothelial venules (HEV) but also in the renal distal tubuli. Here we report that L-selectin-reactive molecules in the kidney are chondroitin sulfate and heparan sulfate proteoglycans of 500–1000 kDa, unlike those in HEV bearing sialyl Lewis X-like carbohydrates. Binding of L-selectin to these molecules was mediated by the lectin domain of L-selectin and required divalent cations. Binding was inhibited by chondroitinase and/or heparitinase but not sialidase. Thus, L-selectin can recognize chondroitin sulfate and heparan sulfate glycosaminoglycans structurally distinct from sialyl Lewis X-like carbohydrates.

【 授权许可】

Unknown