FEBS Letters | |
Identification and characterization of ligands for L‐selectin in the kidney. II. Expression of chondroitin sulfate and heparan sulfate proteoglycans reactive with L‐selectin | |
Watanabe, Norifumi1  Li, Yong-Fei1  Kawashima, Hiroto1  Miyasaka, Masayuki1  | |
[1] Department of Bioregulation, Biomedical Research Center, Osaka University Medical School 2-2, Yamada-oka, Suita 565-0871, Japan | |
关键词: L-selectin; Kidney; Chondroitin sulfate; Heparan sulfate; Proteoglycan; Sialyl Lewis X; | |
DOI : 10.1016/S0014-5793(99)00046-0 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Ligands for the leukocyte adhesion molecule L-selectin are expressed not only in lymph node high endothelial venules (HEV) but also in the renal distal tubuli. Here we report that L-selectin-reactive molecules in the kidney are chondroitin sulfate and heparan sulfate proteoglycans of 500–1000 kDa, unlike those in HEV bearing sialyl Lewis X-like carbohydrates. Binding of L-selectin to these molecules was mediated by the lectin domain of L-selectin and required divalent cations. Binding was inhibited by chondroitinase and/or heparitinase but not sialidase. Thus, L-selectin can recognize chondroitin sulfate and heparan sulfate glycosaminoglycans structurally distinct from sialyl Lewis X-like carbohydrates.
【 授权许可】
Unknown
【 预 览 】
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RO201912020307239ZK.pdf | 985KB | download |