Hormonal effects on the phosphorylation of glycogen synthase in rat hepatocytes

FEBS Letters

Ariño, Joaquín¹  Mor, Angels¹  Baanante, Isabel V.¹  Fátima, Bosch¹  Guinovart, Joan J.¹ 

[1] Department de Bioquímica, Facultat de Farmàcia, Universitat de Barcelona, Pedralbes, Barcelona 28, Spain

关键词: Glycogen synthase;    Hepatocyte;    Phosphorylation;    CNBr peptide;    Epinephrine;    Vasopressin;    Hormonal effect;    M app;    apparent molecular mass;   

DOI  :  10.1016/0014-5793(84)81334-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effects of epinephrine and vasopressin on the phosphorylation state of glycogen synthase were studied using rat hepatocytes incubated with ³²P. After the incubation with hormones, ³²P-labeled glycogen synthase was isolated using antibodies against rat liver enzyme. The immunoprecipitate showed a single radioactive band (M _app 88 kDa) when subjected to SDS-gel electrophoresis. Both epinephrine and vasopressin inactivated the enzyme and increased the ³²P content of glycogen synthase. Cleavage of the immunoprecipitate with CNBr yielded two major ³²P-labeled fragments of M _app∼27 and 12 kDa. Both hormones increased the ³²P content of both fragments. These results prove that epinephrine and vasopressin increase the phosphate content of the enzyme promoting its phosphorylation at multiple sites.

【 授权许可】

Unknown