Zonal distribution of glycogen synthesis in isolated rat hepatocytes

FEBS Letters

Ferrer, Juan C²  Guinovart, Joan J²  López-Iglesias, Carmen¹  Fernández-Novell, Josep M² 

[1] Serveis Cientı́fico-Tècnics, Universitat de Barcelona, E-08028 Barcelona, Spain;Departament de Bioquı́mica i Biologia Molecular and Institut de Recerca Biomèdica de Barcelona-Parc Cientı́fic de Barcelona, Martı́ i Franquès 1, 7 planta, E-08028 Barcelona, Spain

关键词: Glycogen;    Hepatocyte;    Glycogen synthase;    Electron microscopy;    GS;    glycogen synthase;    LGS;    liver glycogen synthase;    GK;    glucokinase;    PAS;    periodic acid Schiff;   

DOI  :  10.1016/S0014-5793(02)03506-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Incubation of hepatocytes isolated from fasted rats with [¹⁴C]glucose for short periods of time showed that the initial stages of glycogen synthesis occur near the plasma membrane. Incubation with [¹⁴C]glucose followed by cold glucose demonstrated that glycogen synthesis is always active at the hepatocyte periphery and that previously synthesised glycogen moves towards the centre of the cell, while its place is filled by newly synthesised molecules. However, the reverse experiment, incubation with cold glucose before addition of [¹⁴C]glucose, showed that, as glycogen synthesis progresses, it also becomes gradually active in more internal sites of the hepatocyte. These results indicate a spatial order in the synthesis of hepatic glycogen.

【 授权许可】

Unknown