Nuclear glycogen synthase — fact or artifact?

FEBS Letters

Granzow, Christof¹  Spring, Herbert²  Kopun, Marijana¹ 

[1] Institut für Zell- und Tumorbiologie, Deutsches Krebsforschungszentrum, Postfach 101 949, Im Neuenheimer Feld 280, 6900 Heidelberg, FRG;Institut für Experimentelle Pathologie, Deutsches Krebsforschungszentrum, Postfach 101 949, Im Neuenheimer Feld 280, 6900 Heidelberg, FRG

关键词: Ehrlich cell;    Glycogen;    Glycogen synthase;    Nucleus;    Cytochemistry;   

DOI  :  10.1016/0014-5793(82)81043-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

According to Oron [FEBS Lett. (1980) 118, 255–258], nuclear glycogen synthase represents an artifact of preparation in rat liver nuclei. We investigated the nuclei isolated from in vitro growing HD33 ascites cells with exclusively cytoplasmic, and from in vivo growing HD33 Ehrlich—Lettré ascites tumor cells with mainly intranuclear, glycogen deposition. Biochemical and ultracytochemical analyses revealed the complete absence of any contamination of the isolated nuclei by cytoplasmic glycogen particles and associated glycogen synthase activity. The glycogen synthase residing in isolated nuclei of in vivo growing HD33 ascites tumor cells represents a truly nuclear enzyme activity

【 授权许可】

Unknown