期刊论文详细信息
FEBS Letters | |
The glycoprotein 71 of ecotropic Friend murine leukemia virus | |
Schlüter, Michael1  Geyer, Rudolf1  Stirm, Stephan1  Linder, Dietmar1  Schneider, Josef2  Hunsmann, Gerhard2  | |
[1] Biochemisches Institut am Klinikum der Universität, friedrichstrasse 24, D-6300 Giessen FRG;Forschergruppe Tumorimmunologie, Stefan Meier-Str. 8, D-7800 Freiburg, FRG | |
关键词: Glycoprotein; Glycosylation site; Leukemia; Methylation analysis; Oligosaccharide structure; Retrovirus; endo H; endo-β-N-acetyl-D-glucosaminidase H from Streptomyces griseus; GC; gas chromatography; gp; glycoprotein; gp71; gp of apparent M r 71 000; HPLC; high-performance liquid chromatography; rHPLC; reversed-phase HPLC; MS; mass spectrometry; MuLV; murine leukemia virus; F-MuLV; Friend strain of MuLV; PTH amino acids; phenylthiohydantoin amino acids; SA; sialic acid; | |
DOI : 10.1016/0014-5793(84)80317-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The glycoprotein from Friend murine leukemia virus was digested with protease from Staphylococcus aureus V8. A glycopeptide comprising the N-terminal glycosylation site (Asn-12) was isolated from the mixture of fragments and analyzed by amino acid sequencing and methylation-capillary gas chromatography—mass spectrometry before and after treatment with sialidase from Vibrio cholerae. Asn-12 was thus found to be substituted by a family of partially sialylated, fucosylated, and intersected glycoprotein N-glycans of the hybrid type.
【 授权许可】
Unknown
【 预 览 】
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