The glycoprotein 71 of ecotropic Friend murine leukemia virus

FEBS Letters

Schlüter, Michael¹  Geyer, Rudolf¹  Stirm, Stephan¹  Linder, Dietmar¹  Schneider, Josef²  Hunsmann, Gerhard² 

[1] Biochemisches Institut am Klinikum der Universität, friedrichstrasse 24, D-6300 Giessen FRG;Forschergruppe Tumorimmunologie, Stefan Meier-Str. 8, D-7800 Freiburg, FRG

关键词: Glycoprotein;    Glycosylation site;    Leukemia;    Methylation analysis;    Oligosaccharide structure;    Retrovirus;    endo H;    endo-β-N-acetyl-D-glucosaminidase H from Streptomyces griseus;    GC;    gas chromatography;    gp;    glycoprotein;    gp71;    gp of apparent M r 71 000;    HPLC;    high-performance liquid chromatography;    rHPLC;    reversed-phase HPLC;    MS;    mass spectrometry;    MuLV;    murine leukemia virus;    F-MuLV;    Friend strain of MuLV;    PTH amino acids;    phenylthiohydantoin amino acids;    SA;    sialic acid;   

DOI  :  10.1016/0014-5793(84)80317-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The glycoprotein from Friend murine leukemia virus was digested with protease from Staphylococcus aureus V8. A glycopeptide comprising the N-terminal glycosylation site (Asn-12) was isolated from the mixture of fragments and analyzed by amino acid sequencing and methylation-capillary gas chromatography—mass spectrometry before and after treatment with sialidase from Vibrio cholerae. Asn-12 was thus found to be substituted by a family of partially sialylated, fucosylated, and intersected glycoprotein N-glycans of the hybrid type.

【 授权许可】

Unknown