Localization and characterization of the glycosylation site of human pancreatic elastase 1

FEBS Letters

Sziegoleit, Andreas²  Wendorf, Petra²  Geyer, Rudolf¹  Linder, Dietmar¹ 

[1] Biochemisches Institut am Klinikum der Universität, Friedrichstr. 24, D-6300 Giessen, FRG;Institut für Medizinische Mikrobiologie der Universität, Frankfurter Str. 107, Germany

关键词: Elastase 1;    Glycoprotein;    Glycosylation site;    Oligosaccharide composition;    (Human pancreas);    E1;    human pancreatic elastase 1;    PTH;    phenylthiohydantoin;    rHPLC;    reversed-phase high-performance liquid chromatography;    S;    tryptic peptide containing the sequence …Asn-X-Ser/Thr…;    TPCK;    tosylnitrophenyl chloromethyl ketone;   

DOI  :  10.1016/0014-5793(89)80640-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Crystalline elastase 1 from human pancreas was digested with trypsin. Two peptides, containing the potential N-glycosylation sites at Asn-86 and Asn-125, were isolated and analyzed by amino acid analysis, sequencing and carbohydrate component analysis. The results demonstrate that only Asn-86 is glycosylated.

【 授权许可】

Unknown