期刊论文详细信息
FEBS Letters | |
Localization and characterization of the glycosylation site of human pancreatic elastase 1 | |
Sziegoleit, Andreas2  Wendorf, Petra2  Geyer, Rudolf1  Linder, Dietmar1  | |
[1] Biochemisches Institut am Klinikum der Universität, Friedrichstr. 24, D-6300 Giessen, FRG;Institut für Medizinische Mikrobiologie der Universität, Frankfurter Str. 107, Germany | |
关键词: Elastase 1; Glycoprotein; Glycosylation site; Oligosaccharide composition; (Human pancreas); E1; human pancreatic elastase 1; PTH; phenylthiohydantoin; rHPLC; reversed-phase high-performance liquid chromatography; S; tryptic peptide containing the sequence …Asn-X-Ser/Thr…; TPCK; tosylnitrophenyl chloromethyl ketone; | |
DOI : 10.1016/0014-5793(89)80640-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Crystalline elastase 1 from human pancreas was digested with trypsin. Two peptides, containing the potential N-glycosylation sites at Asn-86 and Asn-125, were isolated and analyzed by amino acid analysis, sequencing and carbohydrate component analysis. The results demonstrate that only Asn-86 is glycosylated.
【 授权许可】
Unknown
【 预 览 】
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RO201912020292081ZK.pdf | 218KB | download |