Genetic diversity of HA1 domain of heammaglutinin gene of influenza A(H1N1)pdm09 in Tunisia

Virology Journal

Amine Slim²  Inmaculada Casas¹  Maria Teresa Cuevas¹  Juan Ledesma¹  Francisco Pozo¹  Mohamed Ali Ben Hadj Kacem²  Awatef El Moussi³ 

[1] National Influenza Centre-Madrid, Influenza and Respiratory Viruses Laboratory, Instituto de Salud Carlos III, Madrid, Spain;Faculty of Medicine of Tunis, Tunis El Manar University, Tunis El Manar, Tunisia;National Influenza Centre-Tunis, Unit Virology, Microbiology Laboratory, Charles Nicolle’s Hospital, Tunis, Tunisia

关键词: Phylogeny;    Influenza A(H1N1)pdm09;    Heammaglutinin;    Glycosylation site;    Antigenic site;   

Others  :  1150312
DOI  :  10.1186/1743-422X-10-150

 received in 2012-10-28, accepted in 2013-03-25,  发布年份 2013

【 摘 要 】

We present major results concerning isolation and determination of the nucleotide sequence of hemagglutinin (HA1) of the pandemic (H1N1)pdm09 influenza viruses found in Tunisia. Amino acid analysis revealed minor amino acid changes in the antigenic or receptor-binding domains. We found mutations that were also present in 1918 pandemic virus, which includes S183P in 4 and S185T mutation in 19 of 27 viruses analyzed from 2011, while none of the 2009 viruses carried these mutations. Also two specific amino acid differences into N-glycosylation sites (N288T and N276H) were detected. The phylogenetic analysis revealed that the majority of the Tunisian isolates clustered with clade A/St. Petersburg/27/2011 viruses characterized by D97N and S185T mutations. However it also reveals a trend of 2010 strains to accumulate amino acid variation and form new phylogenetic clade with three specific amino acid substitutions: V47I, E172K and K308E.

【 授权许可】

   
2013 El Moussi et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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