Heparin inhibits the activity of protein phosphatase‐1

FEBS Letters

Bot, György¹  Erdődi, Ferenc¹  Gergely, Pál¹ 

[1] Institute of Medical Chemistry, University School of Medicine, Bem tér 18/B, H-4026 Debrecen, Hungary

关键词: Glycogen metabolism;    Protein phosphatase-1;    Phosphorylase a;    Heparin;    Inhibitor-1;    Heparin—Sepharose;   

DOI  :  10.1016/0014-5793(84)80286-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Heparin inhibited the dephosphorylation of rabbit skeletal muscle or liver phosphorylase a by protein phosphatase-1. Other glycosaminoglycans (chondroitin sulfates) and their constituents were found to be without effect. The chromatography of a partially purified phosphatase preparation on heparin—Sepharose CL-6B resulted in a fraction that did not bind to the matrix and its activity was not inhibited by heparin or inhibitor-1. The phosphatase bound to heparin—Sepharose was eluted by 0.2 M NaCl and was inhibited by heparin or inhibitor-1.

【 授权许可】

Unknown