The ammonium sulfate activation of phosphorylase b

FEBS Letters

Papageorgiou, A.C.¹  Xenakis, A.¹  Leonidas, D.D.¹  Oikonomakos, N.G.¹  Bem, F.²  Cazianis, C.T.¹ 

[1] Biological Research Center, The National Hellenic ResearchFoundation, 48 Vas. Constantinou Ave., Athens 11635, Greece;Laboratory of Virology, Benaki Phytopathological Institute, 2 Ekalis, Kifissia 14561, Greece

关键词: Phosphorylase b;    Phosphorylase a;    Activation;    Electron spin resonance;    Tetramerization;    Structure-function;    glucose-1-P;    α-D-glucopyranose-1-phosphate;    glucose-6-P;    glucose-6-phosphate;    4-(2-iodoacetamido)2;    2;    6;    6-tetramethyl piperidinyloxyl;    iodoacetamide spin label;    ESR;    electron spin resonance;    glycogen phosphorylase;    1;    4-α-D-glucan;    orthophosphate o-glucosyltransferase (EC 2.4.1.1);   

DOI  :  10.1016/0014-5793(90)80627-U

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The ammonium sulfate activation of phosphorylase b has been studied. Ammonium sulfate, when present in high concentrations, induces properties of phosphorylase a in phosphorylase b, such as an enhanced affinity for AMP, a reversal of the glucose-6-P inhibition and enzyme tetramerization. The data are consistent with the interpretation that sulfates bind to the Ser-14 site and the sulfate-protein interactions at this site are responsible for activation of phosphorylase b.

【 授权许可】

Unknown