Cotransport of proline and Li+ in Escherichia coli

FEBS Letters

Yamane, Yoshiko¹  Shiota, Sumiko¹  Tsuchiya, Tomofusa¹  Kawasaki, Takashi² 

[1] Department of Microbiology, Faculty of Pharmaceutical Sciences, Okayama University, Okayama 700 Japan;Department of Biochemitry, School of Medicine, Hiroshima University, Hiroshima 734, Japan

关键词: Li+;    Proline;    Cotransport;    Li+ electrode;    Kinetic parameters;    Temperature effect;   

DOI  :  10.1016/0014-5793(84)80272-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Uptake of Li⁺ induced by the addition of proline to a cell suspension of Escherichia coli was detected using an Li⁺-selective electrode. This Li⁺ uptake was inhibited by L-azetidine 2-carboxylic acid, a competitive inhibitor of the proline transport system. Thus, direct evidence for Li⁺-proline cotransport via the proline transport system was obtained. Kinetic parameters of the Li⁺ uptake were determined.

【 授权许可】

Unknown