Dependence on pH of substrate binding to lactose carrier in Escherichia coli cytoplasmic membranes

FEBS Letters

Yamato, I.¹  Rosenbusch, J.P.¹ 

[1] Department of Microbiology, Biozentrum, University of Basel, Basel, CH-4056 Basel, Switzerland

关键词: Cotransport;    Galactoside;    Ligand binding;    Permease;    Protonmotive force;    Uncoupling;   

DOI  :  10.1016/0014-5793(83)80352-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Lactose permease in Escherichia mediates proton-substrate cotransport. The molecular mechanism of this process is not understood. We examined the effect of proton concentration on the binding of a substance analogue to the carrier. The dissociation constant of p-nitrophenyl-α-galactoside from the carrier was dependent on pH, with an apparent pK _a of 9.7.

【 授权许可】

Unknown