FEBS Letters | |
Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes | |
Adolph, Kenneth W.1  | |
[1] Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN USA | |
关键词: Nonhistone; Chromatin; Metaphase chromosome; HeLa cell; Antiserum; Electrophoresis; SDS; sodium dodecyl sulfate; PMSF; phenylmethylsulfonyl fluoride; NP40; Nonidet P40; Hepes; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; BSA; bovine serum albumin; | |
DOI : 10.1016/0014-5793(84)80171-4 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with 125I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with 125I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.
【 授权许可】
Unknown
【 预 览 】
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