Conservation of interphase chromatin nonhistone antigens as components of metaphase chromosomes

FEBS Letters

Adolph, Kenneth W.¹ 

[1] Department of Biochemistry, University of Minnesota Medical School, Minneapolis, MN USA

关键词: Nonhistone;    Chromatin;    Metaphase chromosome;    HeLa cell;    Antiserum;    Electrophoresis;    SDS;    sodium dodecyl sulfate;    PMSF;    phenylmethylsulfonyl fluoride;    NP40;    Nonidet P40;    Hepes;    4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid;    BSA;    bovine serum albumin;   

DOI  :  10.1016/0014-5793(84)80171-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The degree of conservation of HeLa interphase chromatin nonhistone antigens among the nonhistones of isolated metaphase chromosomes was determined with immunological procedures. Proteins were separated on SDS-polyacrylamide gels and electrophoretically transferred to diazophenylthioether (DPT)-paper, which was then overlaid with antiserum to chromatin from interphase nuclei. The bound antibodies were detected with ¹²⁵I-labeled protein A. Alternatively, polyacrylamide gels were directly overlaid with antiserum and with ¹²⁵I-protein A. Densitometry of autoradiograms and stained gels revealed the degree of conservation of nonhistone antigenic determinants from interphase to metaphase to be over 90% for chromatin.

【 授权许可】

Unknown