Localization of proteins forming the outer surface of isolated metaphase chromosomes

FEBS Letters

Belyaev, Nickolaj D.²  Sidorov, Victor N.¹  Dubrovskaya, Veronika A.²  Kim, Andrej A.¹  Budker, Vladimir G.²  Kiseleva, Elena V.³ 

[1] Institute of Nuclear Physics, Academy of Sciences of Uzbek SSR, Taschkent, settlement Ulugbek, 702132, USSR;Novosibirsk Institute of Bioorganic Chemistry, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR;Novosibirsk Institute of Cytology and Genetics, Siberian Division of the USSR Academy of Sciences, Novosibirsk 630090, USSR

关键词: Metaphase chromosome;    Non-histone protein;    Scaffold;    TCA;    trichloroacetic acid;    EDTA;    ethylendiamine-tetraacetic acid;    topo II;    topoisomerase II;    HEPES;    N-2-hydroxyethyl-piperazine-N′-2-ethanesulfonic acid;   

DOI  :  10.1016/0014-5793(92)80323-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The outer surface of isolated metaphase chromosomes has been investigated by a method of thermally activated tritium labelling. We show that both chromosomal proteins and DNA are tritium-labelled. Fractionation of the chromosomal proteins reveals that scaffold proteins are the most labelled in condensed and EDTA-decondensed chromosomes. Exposition of some scaffold proteins on the outer surface of metaphase chromosomes is suggested.

【 授权许可】

Unknown