期刊论文详细信息
FEBS Letters | |
Decarboxylated bone Gla‐protein as a substrate for hepatic vitamin K‐dependent carboxylase | |
Hendrix, Hans1  Vermeer, Cees1  de Boer-van den Berg, Marian A.G.1  Soute, Berry A.M.1  | |
[1] Department of Biochemistry, University of Limburg, P.O. Box 616, Maastricht, The Netherlands | |
关键词: Carboxylase; Vitamin K; Warfarin; γ-Carboxyglutamic acid; Osteocalcin; | |
DOI : 10.1016/0014-5793(84)80005-8 | |
学科分类:生物化学/生物物理 | |
来源: John Wiley & Sons Ltd. | |
【 摘 要 】
Bovine bone Gla-protein (B.G.P.) was prepared and decarboxylated into descarboxy-B.G.P. (d-B.G.P.). The latter was purified and identified as decarboxylated osteocalcin. Both crude and purified d-B.G.P. are good substrates for vitamin K-dependent carboxylase. Because the K m of this enzyme for d-B.G.P. is low, the latter is a better substrate than the frequently used pentapeptide F L E E L or exogenous protein substrates such as descarboxyprothrombin.
【 授权许可】
Unknown
【 预 览 】
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RO201912020285030ZK.pdf | 447KB | download |