Decarboxylated bone Gla‐protein as a substrate for hepatic vitamin K‐dependent carboxylase

FEBS Letters

Hendrix, Hans¹  Vermeer, Cees¹  de Boer-van den Berg, Marian A.G.¹  Soute, Berry A.M.¹ 

[1] Department of Biochemistry, University of Limburg, P.O. Box 616, Maastricht, The Netherlands

关键词: Carboxylase;    Vitamin K;    Warfarin;    γ-Carboxyglutamic acid;    Osteocalcin;   

DOI  :  10.1016/0014-5793(84)80005-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Bovine bone Gla-protein (B.G.P.) was prepared and decarboxylated into descarboxy-B.G.P. (d-B.G.P.). The latter was purified and identified as decarboxylated osteocalcin. Both crude and purified d-B.G.P. are good substrates for vitamin K-dependent carboxylase. Because the K _m of this enzyme for d-B.G.P. is low, the latter is a better substrate than the frequently used pentapeptide F L E E L or exogenous protein substrates such as descarboxyprothrombin.

【 授权许可】

Unknown