A conjugate of the A1 peptide of chloera toxin and the lectin of Wisteria floribunda that activates the adenylate cyclase of intact cells

FEBS Letters

van Heyningen, Simon¹ 

[1] Department of Biochemistry, University of Edinburgh, Hugh Robson Building, George Square, Edinburgh EH8 9XD, Scotland

关键词: Cholera toxin;    Adenylate cyclase;    Lectin;    Hybrid;    Conjugate;   

DOI  :  10.1016/0014-5793(83)80035-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The active A1 peptide of cholera toxin was linked by a disulphide bond to the lectin of Wisteria floribunda. The resulting conjugate activated the adenylate cyclase of intact U937 or K562 cells at the same concentrations as native toxin did, but to a greater extent. Activation was inhibited by N-acetyl-D-galactosamine or by antisera to the lectin or peptide. The characteristic lag phase between addition of toxin to cells and activation of cyclase was not found with the conjugate or with free A1 peptide.

【 授权许可】

Unknown