Effect of antipsychotic drugs on the molecular action of cholera toxin in rabbit intestinal epithelial cells

FEBS Letters

Longbottom, David¹  van Heyningen, Simon¹ 

[1] Department of Biochemistry, Hugh Robson Building, University of Edinburgh, George Square, Edinburgh EH8 9XD, UK

关键词: Antipsychotic drug;    Cholera toxin;    Adenylate cyclase;    ADP-ribosylation;   

DOI  :  10.1016/0014-5793(90)80444-N

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Antipsychotic drugs of known antidiarrhoeal and anticalmodulin activity inhibited the cholera-toxin-catalysed ADP-ribosylation of proteins of M _r, 37000,40000 and 45000 (thought to be regulatory components of the adenylate cyclase complex) that was previously shown to occur in plasma membranes from rabbit intestinal epithelial cells [(1989) Biochim. Biophys. Acta 1014, 289-297]. There was no obvious correlation between the different activities of the drugs. The drugs also inhibited adenylate cyclase activity, but in this case the inhibition correlated well with the known IC₅₀ values of the drugs for anticalmodulin activity and with their antidiarrhoeal activities.

【 授权许可】

Unknown