Receptor‐mediated ADP‐ribosylation of a phospholipase C‐stimulating G protein

FEBS Letters

Jakobs, Karl H.¹  Gierschik, Peter¹ 

[1] Pharmakologisches Institut der Universität Heidelberg, Im Neuenheimer Feld 366, D-6900 Heidelberg, FRG

关键词: G protein;    ADP-ribosylation;    Cholera toxin;    Formyl peptide;    Neutrophil;    Phospholipase C;    G protein;    guanine nucleotide-binding protein;    FMLP;    N-formyl-methionyl-leucyl-phenylalanine;    GTP[S];    guanosine-5′-O-(3-thiotriphosphate);    GppNHp;    guanylyl-imidodiphosphate;   

DOI  :  10.1016/0014-5793(87)80451-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In membranes of myeloid differentiated HL 60 cells, the chemotactic peptide FMLP stimulates phospholipase C via a pertussis toxin-sensitive G protein. FMLP markedly stimulates the cholera toxin-dependent ADP-ribosylation of a 40 kDa protein in these membranes. This effect of FMLP is inhibited by GTP and GTP[S], and is almost completely abolished in membranes of pertussis toxin-pretreated HL 60 cells. Treatment of HL 60 membranes with cholera toxin and NAD markedly inhibits FMLP-stimulated high affinity GTPase. These results suggest that a 40 kDa G protein sensitive to both pertussis and cholera toxin functionally interacts with the formyl peptide receptor of HL 60 cells and, thus, very likely is the G protein that stimulates phospholipase C in this system.

【 授权许可】

Unknown