Enzyme specificity: base recognition and hydrolysis of RNA by ribonuclease A

FEBS Letters

Borkakoti, Nivedita¹ 

[1] Laboratory of Molecular Biology, Department of Crystallography, Birkbeck College, Malet Street, London WC1E 7HX, England

关键词: Substrate specificity;    Base recognition;    Charge complementarity;    X-ray diffraction;    Conformational flexibility;    Protein-nucleic acid interaction;   

DOI  :  10.1016/0014-5793(83)80789-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The substrate specificity of pancreatic ribonuclease A is discussed in light of observations based on accurate X-ray structure analysis of several enzyme—nucleotide complexes. A hypothesis for protein—nucleic acid recognition is presented which proposes that: (a) pyrimidine bases in RNA are recognised by ribonuclease due to the charge complementarity of two groups (the amide nitrogen and the side chain oxygen (OG) of threonine 45) of the protein and relevant atoms in the heterocyclic base (O2 and N3 in pyrimidine nucleotides); (b) interaction of the protein with the ribose moiety of the nucleotides is non-specific; and (c) conformational flexibility in the region of the scissile PO bond is provided by different locations of the phosphoryl oxygens, rather than by an overall translation of the phosphate moiety.

【 授权许可】

Unknown