Fate of newly synthesized histones in G1 and G0 cells

FEBS Letters

Bonner, William M.¹  Perry, Linda J.¹  Wu, Roy S.¹ 

[1] Laboratory of Molecular Pharmacology, Developmental Therapeutics Program, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health, Bldg. 37 5D17, Bethesda, MD 20205, USA

关键词: Histone;    Chromatin;    Nucleosome Gel;    Cell cycle;   

DOI  :  10.1016/0014-5793(83)81070-9

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We have shown that quiescent cells as well as those in the G₁ phase of the cell cycle synthesize histones at a reduced but significant rate. Now, we show that the histones synthesized during G₀ and G₁ are stably incorporated into nuclei soon after synthesis. Micrococcal nuclease digestion of nuclei isolated from cells in G₀ and G₁ revealed that the specific histone variants synthesized in these different physiological states are found associated with DNA as nucleosomes. Nucleosomes were separated by polyacrylamide gel electrophoresis in a reducing buffer so that histone spot morphology, particularly that of the H3s was improved.

【 授权许可】

Unknown