The translation of the messenger for the poly(A)‐binding protein‐associated with translated mRNA is suppressed

FEBS Letters

Akhayat, Omar¹  Vincent, Alain¹  Person, Anne¹  Goldenberg, Samuel¹  Scherrer, Klaus¹ 

[1] Institut Jacques Monod, Université Paris VII - Tour 43, 75251 Paris Cedex 05, France

关键词: Cytoplasmic repression;    Poly(A)-binding protein;    mRNA translation;    Messenger suppression;    Protein synthesis;    (Duck erythroblasts);    pre-mRNA;    pre-messenger RNA;    mRNA;    messenger RNA;    ScRNA;    small cytoplasmic RNA;    mRNP;    complex messenger RNA-protein;    EAT;    Ehrlich ascites tumor;    kDa;    kilodalton;   

DOI  :  10.1016/0014-5793(83)81042-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

In vivo protein synthesis in duck erythroblasts was compared to in vitro translation of polyribosomal and free cytoplasmic mRNA. The in vivo study showed the absence of de novo synthesis of the M _r 73 000 poly(A)-binding protein found associated with all polyribosomal mRNA. In vitro translation demonstrated that the mRNA for this protein is absent from the polyribosomal mRNA fraction but constitutes a medium frequency messenger among the repressed free mRNA. This result confirms the existence of a qualitative translational control in terminal differentiating duck erythroblasts leading eventually to the arrest of the protein synthesizing machinery.

【 授权许可】

Unknown