Intracellular pH and free calcium changes in single cells using quene 1 and quin 2 probes and fluorescence microscopy

FEBS Letters

Robin Hesketh, T.²  Johnson, Pauline¹  Metcalfe, James C.²  Rogers, Jane²  Garland, Peter B.¹  Beaven, Michael A.²  Smith, Gerry A.² 

[1] Department of Biochemistry, Medical Sciences Institute, University of Dundee, Dundee DD1 4HN, Scotland;Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Free Ca2+;    Intracellular pH;    Thymocyte;    2H3 cell;    Antigenic stimulation;    Fluorescence microscopy;   

DOI  :  10.1016/0014-5793(83)80722-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Photometric fluorescence microscopy has been used to measure intracellular pH (pH_i) and free calcium concentrations ([Ca]_i) in individual mouse thymocytes and 2H3 rat basophil leukaemic cells containing indicators for pH (quene 1) or calcium (quin 2). The pH_i and [Ca]_i measurements in individual 2H3 cells and mouse thymocytes and their responses to various stimuli were consistent with the corresponding data obtained from suspensions of these cells measured in a spectrofluorimeter. Photometric fluorescence microscopy of these indicators in individual cells provides a sensitive and fast method of following pH_i and [Ca]_i responses in individual cells.

【 授权许可】

Unknown