Some mitogens cause rapid increases in free calcium in fibroblasts

FEBS Letters

Morris, Jonathan D.H.¹  Metcalfe, James C.¹  Taylor, Michael V.¹  Hesketh, T.Robin¹  Smith, Gerry A.¹ 

[1] Department of Biochemistry, University of Cambridge, Tennis Court Road, Cambridge CB2 1QW, England

关键词: Free Ca2+;    Quin2;    3T3 fibroblast;    Growth factor;    Mitogenic stimulation;   

DOI  :  10.1016/0014-5793(84)80316-6

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Quiescent 3T3 fibroblasts grown on microcarrier beads and loaded with the [Ca²⁺] indicator quin2 had a cytosolic free Ca²⁺ concentration ([Ca²⁺]_i) of 154 ± 11 nM (SE; n = 32). Stimulation with the mitogens vasopressin, epidermal growth factor (EGF) or prostaglandin F_2α (PGF_2α) caused a very rapid increase in [Ca²⁺]_i to a maximum of 200–500 nM after 60–90 s. [Ca²⁺]_i declined thereafter to a level above that in quiescent cells which was maintained for at least 15 min. In contrast no immediate effects on [Ca²⁺]_i were detected after the addition of the mitogens insulin or 12-O-tetradecanoylphorbol 13-acetate (TPA). These studies indicate that early changes in [Ca²⁺]_i may be involved in the action on fibroblasts of some, but not all, mitogens.

【 授权许可】

Unknown