Using two-photon excitation methods for determination of Ca2+ in contamination with protein

International Journal of Physical Sciences

A. S. M. Noor¹ 

关键词: Free Ca2+;    indo-1;    two-photon excitation methods.;   

DOI  :  10.5897/IJPS11.482

学科分类：物理（综合）

来源: Academic Journals

【 摘 要 】

Three emission wavelengths from two-photon excitationtechniques were useto determinefreeCa2+using a new analytical calculation. This was achieved by considering theinteraction of protein withfreeCa2+and indo-1 in protein contaminated sample. The emissions obtained from the excitation with the dissociation constants were used in the calculation. Agreement values of the Ca2+from the calculation with the known Ca2+buffer solution usedforthe measurement was obtained for various Ca2+and protein concentration. The analytical analysis shows that dissociation constantsfor eachbinding’sdeviation errors are small to affect free Ca2+determination. This would provide an accurate measurement of free Ca2+in biological cells than the typical two emission wavelength processes. Furthermore, using two-photon excitation methods, deep inside cells observation of free Ca2+are possible using the methods introduced.

【 授权许可】

CC BY