Polymorphism of human pituitary lutropin (LH)

FEBS Letters

Rinne, G.¹  Weise, H.C.¹  Graesslin, D.¹  Lichtenberg, V.¹ 

[1] Abteilung für klinische und experimentelle Endokrinologie, Universitäts-Frauenklinik, Martinistr. 52, 2000 Hamburg-Eppendorf, FRG

关键词: Human lutropin (LH);    Pituitary;    Isohormone;    Isoelectric focusing;    Microheterogeneity;    LH;    luteinizing hormone;    FSH;    follicle-stimulating hormone;    IEF;    isoelectric focusing;    NANA;    N-acetyl neuraminic acid;   

DOI  :  10.1016/0014-5793(83)80423-2

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The complete microheterogeneous system of human pituitary lutropin was demonstrated by gel isoelectric focusing. In addition 7 LH isohormones could be isolated by preparative column focusing and characterized with respect to physicochemical, biological and immunological properties. The in vivo biopotencies ranged from 4.50–11.50 IU/mg, the in vitro bioactivity was from 1.20–10.10 IU/mg, and the immunological activity was from 3.10–7.55 IU/mg. The sialic acid content was found to be 1.8–3.2%. Treatment with neuraminidase resulted in a shift of all bands to the alkaline region, however the 7 LH forms were still present.

【 授权许可】

Unknown