Polymorphism of human pituitary lutropin (LH)

FEBS Letters

Bettendorf, G.¹  Weise, H.C.¹  Graesslin, D.¹  Lichtenberg, V.¹ 

[1] Abteilung für klinische und experimentelle Endokrinologie, Universitäts-Frauenklinik, Martinistr. 52, D-2000 Hamburg 20, FRG

关键词: Human lutropin;    Isohormone;    Leydig cell;    Receptor binding;    cAMP production;    Testosterone production;    cAMP;    adenosine 3′;    5′-cyclic phosphoric acid;    hCG;    human chorionic gonadotropin;    hLH;    human luteinizing hormone;    MIX;    1-methyl-3-isobutylxanthine;    MEM;    Eagle's minimum essential medium;    RRA;    radioreceptor assay;   

DOI  :  10.1016/0014-5793(84)80281-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The effect of the 7 different molecular forms of human pituitary lutropin (LH) on mouse Leydig cells as a model of a target organ were studied. The hormone functions have been characterized by receptor binding, cAMP accumulation and testosterone production. One important finding was the similar intrinsic in vitro biological activity for all isohormones. Quantitatively, however, the potencies of the 7 hormone forms did not correlate with the activities obtained by radioimmunoassay: there was a dramatic decrease of receptor binding activity and biological activity compared to immunoactivity from the more alkaline to the more acidic LH isohormones.

【 授权许可】

Unknown