Identification and characterization of variant forms of the gastrin‐releasing peptide (GRP)

FEBS Letters

Mutt, V.¹  McDonald, T.J.²  Jörnvall, H.³  Tatemoto, K.¹ 

[1] Department of Biochemistry II, Karolinska Institutet, Stockholm, Sweden;Department of Medicine, University of Western Ontario, London, Canada;Department of Chemistry I, Karolinska Institutet, Stockholm, Sweden

关键词: Gastrin-releasing peptide (GRP);    Bombesin;    Gastrin release;    HPLC;    Ion-exchange chromatography;    β-Aspartyl shift;   

DOI  :  10.1016/0014-5793(83)80527-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Porcine intestinal gastrin-releasing peptide (GRP) has been demonstrated to be structurally identical to the previously characterized gastric GRP. Ion-exchange and high-performance liquid chromatography of porcine intestinal extracts have identified two variant GRP forms. Studies on one of these variant forms suggest that a β-aspartyl shift has occurred in the Asn—His structure of GRP; such a modification in an Asn—His structure occurring in a natural peptide or protein has not been previously reported. This variant GRP, although retaining bioactivity, appears to have reduced potency in elevating canine plasma gastrin levels.

【 授权许可】

Unknown