500 MHz NMR characterization of synthetic bombesin and related peptides in DMSO‐d6 by two‐dimensional techniques

FEBS Letters

Di Bello, Carlo²  Paolillo, Livio⁴  Trivellone, Enrico¹  Corradini, Maria Grazia⁴  Gozzini, Luigia³  D'Auria, Gabriella⁴  Tonellato, Mauro² 

[1] Institute of Chemistry of Molecules of Biological Interest, CNR, Arcofelice, Naples, Italy;Institute of Industrial Chemistry, University of Padua, Padua, Italy;Farmitalia-Cario Erba spa, Chemical Research and Development, Milan, Italy;Department of Chemistry, University of Naples, Naples, Italy

关键词: Bombesin;    NMR Resonance assignment;   

DOI  :  10.1016/0014-5793(88)80177-7

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The proton NMR characterization of bombesin has been carried out at 500 MHz in DMSO-d₆ using two-dimensional homo- and ¹H-¹³C hetero-correlated techniques. All resonances in the NMR spectra have been assigned and several coupling constants have been measured. The backbone J _αCH-NH coupling constants have constant values that vary between 7.8 and 8.2 Hz and indicate an unfolded structure in DMSO-d₆. Discrepancies with data recently obtained at 300 MHz [(1987) Eur. J. Biochem. 168, 193–199] are discussed.

【 授权许可】

Unknown