EaeI: a restriction endonuclease from Enterobacter aerogenes

FEBS Letters

Brown, Nigel L.¹  Whitehead, Philip R.¹ 

[1] Department of Biochemistry, University of Bristol, Bristol BS8 1TD, England

关键词: Site-specific endonuclease;    DNA sequencing;    Molecular cloning;    DNA methylation;    DNA-protein recognition;    Restriction mapping;   

DOI  :  10.1016/0014-5793(83)80217-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We describe the isolation and characterization of a type II restriction endonuclease from Enterobacter aerogenes. This recognises and cleaves the family of related sequences: ↓ 5′-Py—G—G—C—C—Pu-3′ to generate DNA fragments with 5′-tetranucleotide extensions. EaeI may be useful in molecular cloning experiments, especially in conjunction with other enzymes which generate the same terminal extensions. Potential problems in the methods used to determine the cleavage specificity are discussed.

【 授权许可】

Unknown