Restriction endonucleases from Bifidobacterium bifidum

FEBS Letters

Kiwaki, Mayumi¹  Khosaka, Toshiyuki¹ 

[1] Yakult Central Institute for Microbiological Research, 1796 Yaho, Kunitachi, Tokyo 186, Japan

关键词: Site-specific endonuclease;    Isoschizomer;    DNA sequencing;    5'-GR↓CGYC-3';    5'-Terminal extension Molecular cloning;   

DOI  :  10.1016/0014-5793(84)80980-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Three restriction endonucleases, BbiI, BbiII and BbiIII, have been isolated from Bifidobacterium bifidum. The recognition and cleavage specificity of BbiII was determined to be 5'-GR↓CGYC-3', identical to that of AcyI isolated from a cyanobacterium Anabaena cylindrica. The other two enzymes, BbiI and BbiIII, were found to be isoschizomers of PstI and XhoI, respectively.

【 授权许可】

Unknown