R.SspD5I is a neoschizomer of HphI producing blunt end DNA fragments

FEBS Letters

Zheleznaya, Lyudmila¹  Shiryaev, Sergey²  Matvienko, Nicholas²  Zheleznyakova, Elena² 

[1] Institute of Theoretical and Applied Biophysics, RAS, 142292 Pushchino, Russia;Institute of Protein Research, RAS, 142292 Pushchino, Russia

关键词: Site-specific endonuclease;    Neoschizomer;    Restriction;   

DOI  :  10.1016/S0014-5793(99)00330-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The strain Staphylococcus species D5 produces a restriction enzyme. It is the neoschizomer of HphI endonuclease, which cleaves DNA at a distance of eight nucleotides from the recognition sequences producing blunt end DNA fragments: 5′-GGTGA8N↓-3′ and 3′-CCACT8N↑-5′.

【 授权许可】

Unknown