Synchronization of nuclear DNA synthesis in cultured Daucus carota L. cells by aphidicolin

FEBS Letters

Galli, Maria Grazia²  Nielsen, Erik³  Spadari, Silvio¹  Sala, Francesco¹  Devreux, Marcel³  Magnien, Etienne³  Pedrali-Noy, Guido¹ 

[1] Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Via S. Epifanio 14, Pavia, Italy;Istituto di Scienze Botaniche, Centro di Studio del CNR per la Biologia Cellulare e Molecolare delle Piante, Università di Milano, Italy;Biology Group DG XII Joint Research Centre, Ispra, Italy

关键词: Aphidicolin;    Synchronization;    Cultured plant cell;    Daucus carota;    Nuclear DNA synthesis;    DMSO;    dimethyl-sulfoxide;    SCV;    ml semidented cells/100 ml culture;   

DOI  :  10.1016/0014-5793(83)80148-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

By inhibiting the α-like DNA polymerase, and therefore nuclear DNA synthesis, aphidicolin induces accumulation of suspension cultured carrot cells at the G₁/S boundary of the cell cycle. After a 24-h treatment with the drug the accumulation is complete, affecting all the cycling cells (95% of the population). Upon removal of the inhibitor, all cycling cells immediately resume nuclear DNA synthesis and move synchronously throughout the S-phase.

【 授权许可】

Unknown