Na+‐dependent proline transport in isolated membrane vesicles from the L6 muscle cell line

FEBS Letters

Gagalang, Enriqueta¹  Logan, William J.¹  Klip, Amira¹ 

[1] Div. Neurology, The Hospital for Sick Children, 555 University Ave., Toronto, Ont. M5G 1X8, Canada

关键词: Membrane transport;    Myoblast;    Amino acid transport;    Isolated membrane vesicle;    Muscle cells;    Amino acid-supplementation;    Trans-inhibition;   

DOI  :  10.1016/0014-5793(83)80372-X

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Membrane vesicles of L6 myoblasts were prepared in order to study the amino acid transport system A. The role of the membrane in the adaptive response of transport to amino acid-supplementation was assessed. The membranes, prepared by N₂ cavitation, displayed Na⁺ (but not K⁺)-dependent L-proline uptake. An overshoot of L-[³H]proline uptake was observed after exposure of the vesicles to an inward Na⁺ gradient. Isolated membrane vesicles loaded with 50 μM proline displayed countertransport (stimulation of proline uptake). It is concluded that the adaptive decrease of proline uptake observed in amino acid-supplemented cells cannot be accounted for by trans-inhibition of transport.

【 授权许可】

Unknown