Effect of specific inhibition of gamma‐glutamyl transpeptidase on amino acid uptake by mammary gland of the lactating rat

FEBS Letters

Puertes, Inmaculada R.¹  Montoro, Juan B.¹  Viña, Juan R.¹  Viña, José¹ 

[1] Departamento de Bioquimica y Fisiologia, Facultad de Medicina, Avenida Blasco Ibañez, 17, Valencia-10, Spain

关键词: Gamma-glutamyl transpeptidase (EC 2.3.2.2);    Amino acid transport;    Glutathione;    Gamma-glutamyl cycle;    Mammary gland (rat);    Lactation;   

DOI  :  10.1016/0014-5793(83)80428-1

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

We showed [Biochem. J. (1981) 194, 99–102] that inhibition of γ-glutamyl transpeptidase in vivo with serine-borate decreases amino acid uptake by mammary gland. However, doubts arose about the validity of this inhibitor in metabolic studies because it must be used in very large amounts. New Inhibitors have been isolated, like anthglutin and acivicin, which are effective at low concentrations in vivo. Here, we show that treatment of lactating rats with these substances decreases the transpeptidase activity and the amino acid uptake by the gland. These results support the hypothesis that the γ-glutamyl cycle functions as an amino acid transport system in mammary gland.

【 授权许可】

Unknown