Inhibition of γ‐glutamyl transpeptidase decreases amino acid uptake in human keratinocytes in culture

FEBS Letters

Blay, Pilar³  Castells, Antonio¹  Viña, Juan R.²  Viña, José³  Ramirez, Ana¹ 

[1] Departamento de Dermatología, Facultad de Medicina, Universitat de Valencia, Avenida Blasco Ibañez 17, 46010 Valencia, Spain;Departamento de Bioquímica-Biologín Molecular, Facultad de Medicina, Universitat de Valencia, Avenida Blasco Ibanez 17, 46010 Valencia, Spain;Departamento de Fisiología, Facultad de Medicina, Universitat de Valencia, Avenida Blasco Ibanez 17, 46010 Valencia, Spain

关键词: Amino acid transport;    γ-Glutamyl transpeptidase;    Glutathione;    Cell culture;    Human keratinocyte;   

DOI  :  10.1016/0014-5793(90)81125-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Acivicin inhibits γ-glutamyl transpeptidase activity in human keratinocytes in culture. Treatment of these cells with acivicin produces a decrease in the uptake of L-[U-¹⁴C]alanine, 2-amino-[1-¹⁴C]-isobutyrate, L[U-¹⁴C]leucine and l-aminocyclopentane-l-[¹⁴C]carboxylate. D-[U-¹⁴C]glucose uptake is not affected by the presence of acivicin. These results support, for the first time in vitro, the hypothesis that the γ-glutarml cycle may be involved in amino acid uptake by human cells.

【 授权许可】

Unknown