Vitamin K‐dependent carboxylase: Synthesis of an inhibitor of the glutamyl binding site

FEBS Letters

Goodman, Hedda L.¹  Kawai, Megumi²  Rich, Daniel H.²  Engelke, Jean¹  Suttie, J.W.² 

[1] Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA;School of Pharmacy, College of Agricultural and Life Sciences, University of Wisconsin-Madison, Madison, WI 53706, USA

关键词: Vitamin K;    Carboxylase;    Phosphoserine;    Peptide substrates;    γCarboxyglutamic acid;   

DOI  :  10.1016/0014-5793(83)80486-4

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Liver microsomes contain a vitamin K and O₂-dependent carboxylase that converts peptide-bound glutamyl residues to γ-carboxyglutamyl residues. The peptide Boc-O-phospho—Ser-O-phospho—Ser—Leu-OMe has now been synthesized. This peptide inhibits the carboxylation of endogenous protein precursors by a detergent-solubilized preparation of the carboxylase and is an apparent competitive inhibitor of the carboxylation of Phe—Leu—Glu—Glu—Leu.

【 授权许可】

Unknown